De verhaallijn draait om de leerlingen van klas 3-Z en hun gekke leraar Ginpachi-sensei.
Tomokazu Sugita
Gintoki Sakata / Ginpachi-sensei (voice)
Daisuke Sakaguchi
Shinpachi Shimura (voice)
Rie Kugimiya
Kagura (voice)
Satsuki Yukino
Tae Shimura (voice)
Susumu Chiba
Isao Kondou (voice)
Kazuya Nakai
Toushirou Hijikata (voice)
Kenichi Suzumura
Sougo Okita (voice)
Tetsuharu Ota
Sagaru Yamazaki (voice)
Akira Ishida
Kotarou Katsura (voice)
Fumihiko Tachiki
Yasuzo Hasegawa (voice)
Yu Kobayashi
Ayame Sarutobi (voice)
Fumiko Orikasa
Yagyu Kyubei (voice)
Koji Yusa
Ayumu Tojo (voice)
Yuu Sugimoto
Catherine (voice)
